3ZNX

Crystal structure of the OTU domain of OTULIN D336A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	287
Detector technology	CCD
Collection date	2012-11-03
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	43.807, 72.197, 94.843
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.470 - 1.350
R-factor	0.12349
Rwork	0.122
R-free	0.15894
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3znv
RMSD bond length	0.013
RMSD bond angle	1.462
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.420	1.370
High resolution limit [Å]	1.350	1.350
Rmerge	0.090	0.700
Number of reflections	66908
<I/σ(I)>	9.9	2.4
Completeness [%]	100.0	100
Redundancy	6.1	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		100 MM MES/IMIDAZOLE PH 6.5, 30 MM MGCL2, 30 MM CACL2, 10% PEG 4,000, 20% GLYCEROL