3ZNL
H5 Haemagglutinin in Complex with 6-O-Sulfo-Sialyl-Lewis X (Sulfated Lewis X)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-23 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.980, 101.600, 161.370 |
| Unit cell angles | 90.00, 111.32, 90.00 |
Refinement procedure
| Resolution | 35.700 - 2.500 |
| R-factor | 0.23789 |
| Rwork | 0.237 |
| R-free | 0.25582 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ibx |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.008 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 150.760 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.110 |
| Number of reflections | 64504 |
| <I/σ(I)> | 7.3 |
| Completeness [%] | 70.4 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH 7.0, 0.05 M MGCL2, 28 - 30 % PEG 550 MME |
