3ZMK
Anopheles funestus glutathione-s-transferase epsilon 2 (GSTe2) protein structure from different alelles: A single amino acid change confers high level of DDT resistance and cross resistance to permethrin in a major malaria vector in Africa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.170, 99.670, 89.880 |
| Unit cell angles | 90.00, 99.16, 90.00 |
Refinement procedure
| Resolution | 44.367 - 2.200 |
| R-factor | 0.1701 |
| Rwork | 0.168 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2imi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.049 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.550 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.150 | 0.560 |
| Number of reflections | 254696 | |
| <I/σ(I)> | 11.5 | 5 |
| Completeness [%] | 91.6 | 85.1 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 40 %(V/V) PEG 300 AND 0.1 M PHOSPHATE-CITRATE PH 4.2 |
