3ZM8

Crystal structure of Podospora anserina GH26-CBM35 beta-(1,4)- mannanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-07
Detector	ADSC CCD
Spacegroup name	P 65 2 2
Unit cell lengths	97.490, 97.490, 268.717
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.300 - 2.850
R-factor	0.2088
Rwork	0.206
R-free	0.25730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2QHA 2bvt 2VX4 AND 2WHK
RMSD bond length	0.008
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.850	2.850
Rmerge	0.080	0.650
Number of reflections	18575
<I/σ(I)>	25.6	4.6
Completeness [%]	99.9	100
Redundancy	11.8	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		0.1 M TRIS PH 7.0, 0.2 M NACL, 0.8 M POTASSIUM SODIUM TARTRATE, 1 MM HGCL2