3ZM4
Crystal structure of MEK1 in complex with fragment 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Collection date | 2011-04-27 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 77.805, 77.805, 222.926 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.500 - 2.370 |
| R-factor | 0.2074 |
| Rwork | 0.205 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.500 | 2.500 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.100 | 0.520 |
| Number of reflections | 17174 | |
| <I/σ(I)> | 28.3 | 9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 18.6 | 19.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | Appropriate single crystals were grown using this set-up in PEG 4000 18%, Tris 100mM pH7.7, DMSO 2% and CaCl2 0.2M and were extracted from the low volume drops, cryo-protected in mother liquor with 20% glycerol and flash cooled for synchrotron collection |
