3ZLX
Crystal structure of MEK1 in complex with fragment 18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Collection date | 2011-06-07 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 76.930, 76.930, 221.757 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.490 - 2.200 |
R-factor | 0.2053 |
Rwork | 0.204 |
R-free | 0.23610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.810 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.080 | 0.470 |
Number of reflections | 20598 | |
<I/σ(I)> | 16.3 | 6 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 6.2 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.7 | Appropriate single crystals were grown using this set-up in PEG 4000 18%, Tris 100mM pH7.7, DMSO 2% and CaCl2 0.2M and were extracted from the low volume drops, cryo-protected in mother liquor with 20% glycerol and flash cooled for synchrotron collection |