3ZLW
Crystal structure of MEK1 in complex with fragment 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Collection date | 2011-04-27 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 77.059, 77.059, 221.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.120 - 2.120 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 221.800 | 2.230 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.090 | 0.470 |
| Number of reflections | 23238 | |
| <I/σ(I)> | 18.5 | 4.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.9 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | pH 7.7 |
