3ZLH
Structure of group A Streptococcal enolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-31 |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | P 4 |
| Unit cell lengths | 185.795, 185.795, 56.324 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.750 - 2.900 |
| R-factor | 0.15528 |
| Rwork | 0.153 |
| R-free | 0.20540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w6t |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.730 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.750 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.430 | 1.130 |
| Number of reflections | 43076 | |
| <I/σ(I)> | 4.6 | 1.91 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 1-3.5 M SODIUM/POTASSIUM PHOSPHATE BUFFER (PH 5-7.5); 2% PEG 400 |
