3ZLE
Crystal structure of Toxoplasma gondii sporozoite AMA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 179.190, 155.520, 180.590 |
| Unit cell angles | 90.00, 92.31, 90.00 |
Refinement procedure
| Resolution | 78.040 - 2.350 |
| R-factor | 0.21044 |
| Rwork | 0.209 |
| R-free | 0.24393 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x2z |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.310 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.300 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 391285 | |
| <I/σ(I)> | 10 | 2.1 |
| Completeness [%] | 95.4 | 75.8 |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.0 M SUCCINIC ACID PH 7.0, 100 MM HEPES PH 7.0, 1% PEG MONOMETHYL ETHER 2000 AND 100 MM GLYCINE |
