3ZK1
Crystal structure of the sodium binding rotor ring at pH 5.3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-08-15 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 135.770, 83.900, 150.990 |
Unit cell angles | 90.00, 112.85, 90.00 |
Refinement procedure
Resolution | 29.747 - 2.200 |
R-factor | 0.209 |
Rwork | 0.208 |
R-free | 0.23370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wgm |
RMSD bond length | 0.016 |
RMSD bond angle | 2.097 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.210 | 1.150 |
Number of reflections | 158380 | |
<I/σ(I)> | 8.65 | 1.35 |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 4.5 | 4.31 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.3 | pH 5.3 |