3ZK0
The crystal structure of a Cu(I) metallochaperone from Streptomyces lividans in its apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.600, 47.680, 48.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.160 - 1.700 |
| R-factor | 0.18561 |
| Rwork | 0.183 |
| R-free | 0.23303 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zja |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.771 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.160 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.440 |
| Number of reflections | 11478 | |
| <I/σ(I)> | 8.8 | 1.9 |
| Completeness [%] | 98.0 | 89.5 |
| Redundancy | 3.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 31 % PEG 8K, 0.2 M SODIUM ACETATE AND 0.1 M SODIUM CACODYLATE, PH 6.5 |
