3ZJC
Crystal structure of GMPPNP-bound human GIMAP7 L100Q variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.853, 90.939, 114.551 |
| Unit cell angles | 77.30, 85.23, 89.23 |
Refinement procedure
| Resolution | 32.438 - 3.150 |
| R-factor | 0.2379 |
| Rwork | 0.234 |
| R-free | 0.30520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xtn |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.921 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.280 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.110 | 0.490 |
| Number of reflections | 31271 | |
| <I/σ(I)> | 7.24 | 1.58 |
| Completeness [%] | 95.3 | 93.2 |
| Redundancy | 1.8 | 1.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 19% PEG 3350, 100 MM MOPS PH 6.5. |
