3ZJB
The structure of the TRAF domain of human TRAF4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.620, 85.440, 61.650 |
| Unit cell angles | 90.00, 108.08, 90.00 |
Refinement procedure
| Resolution | 58.600 - 1.840 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.19950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ca9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.600 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.840 |
| Rmerge | 0.080 | 1.290 |
| Number of reflections | 46352 | |
| <I/σ(I)> | 10.4 | 1.04 |
| Completeness [%] | 97.3 | 73.9 |
| Redundancy | 3.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 15% PEG 4000, 0.1M HEPES PH 7.0 |
