3ZJA
The crystal structure of a Cu(I) metallochaperone from Streptomyces lividans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-25 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.480, 47.840, 50.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.320 - 1.480 |
R-factor | 0.18741 |
Rwork | 0.185 |
R-free | 0.22675 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.016 |
RMSD bond angle | 1.800 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.600 | 1.560 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.060 | 0.480 |
Number of reflections | 17951 | |
<I/σ(I)> | 9.5 | 2.2 |
Completeness [%] | 98.1 | 98.8 |
Redundancy | 3.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 30% PEG-MME2000, 0.1M KSCN |