3ZIO

minor-site specific NLS (A28)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	300
Collection date	2010-09-02
Spacegroup name	P 21 21 21
Unit cell lengths	78.940, 90.180, 100.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.740 - 2.100
R-factor	0.1835
Rwork	0.182
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ial
RMSD bond length	0.010
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.800	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.150	0.920
Number of reflections	42561
<I/σ(I)>	10.5	2.2
Completeness [%]	99.8	100
Redundancy	7.2	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.65 M SODIUM CITRATE, 0.1 M HEPES BUFFER (PH 6.5) AND 10 MM DTT