3ZHG
Crystallographic structure of the native mouse SIGN-R1 CRD domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.720, 92.770, 77.060 |
| Unit cell angles | 90.00, 121.66, 90.00 |
Refinement procedure
| Resolution | 27.627 - 1.870 |
| R-factor | 0.2072 |
| Rwork | 0.204 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.073 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.530 | 1.980 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.060 | 0.540 |
| Number of reflections | 52474 | |
| <I/σ(I)> | 11.8 | 1.7 |
| Completeness [%] | 93.0 | 72 |
| Redundancy | 5.7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | CRYSTALS GROWN BY MIXING 1 UL OF CRD_SIGN-R1 (4 MG/ML IN 20 MM TRIS/HCL PH 7.5 AND 100 MM NACL) WITH 1UL OF PRECIPITANT CONSISTING IN 0.1 M BISTRIS PH 5.5 AND 1.6 M (NH4)2SO4. |
