3ZFW
Crystal structure of the TPR domain of kinesin light chain 2 in complex with a tryptophan-acidic cargo peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 87.810, 90.860, 94.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.420 - 2.900 |
| R-factor | 0.2042 |
| Rwork | 0.202 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ceq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.420 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.070 | 0.700 |
| Number of reflections | 17034 | |
| <I/σ(I)> | 10.5 | 2.4 |
| Completeness [%] | 99.0 | 98.2 |
| Redundancy | 5.6 | 5.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.10 M MES PH 6.5, 0.2 L-PROLINE, 7% PGA |
