3ZFQ
Crystal structure of product-like, processed N-terminal protease Npro with mercury
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-03-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.640, 41.230, 43.210 |
| Unit cell angles | 90.00, 114.15, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.650 |
| R-factor | 0.21127 |
| Rwork | 0.208 |
| R-free | 0.27570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zfn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.201 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.140 | 0.450 |
| Number of reflections | 4228 | |
| <I/σ(I)> | 7.6 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100MM NAACETATE, PH 8.5 50% PEG6000 |
