3ZFD
Crystal Structure of the Kif4 Motor Domain Complexed With Mg-AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-04-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.718, 62.718, 167.524 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.350 - 1.710 |
| R-factor | 0.16763 |
| Rwork | 0.166 |
| R-free | 0.19483 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hqd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.425 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.350 | |
| High resolution limit [Å] | 1.710 | 1.700 |
| Rmerge | 0.090 | 0.490 |
| Number of reflections | 37057 | |
| <I/σ(I)> | 21.1 | 3.4 |
| Completeness [%] | 99.3 | 95 |
| Redundancy | 11.8 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.5 |
