3ZF0
Phage dUTPases control transfer of virulence genes by a proto-oncogenic G protein-like mechanism. (Staphylococcus bacteriophage 80alpha dUTPase D81A mutant with dUpNHpp).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 87.120, 87.120, 87.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.300 - 2.900 |
| R-factor | 0.24255 |
| Rwork | 0.242 |
| R-free | 0.27463 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.996 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.300 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.070 | 0.350 |
| Number of reflections | 5103 | |
| <I/σ(I)> | 17.6 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.5 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2-8% TERT-BUTANOL, 0.1M TRIS (PH 8.5), 30-50% MPD OR PEG400. |
