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3ZE4

Crystal structure of the integral membrane diacylglycerol kinase - wild-type

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 23-ID-B
Synchrotron siteAPS
Beamline23-ID-B
Temperature [K]100
Detector technologyCCD
Collection date2012-06-13
DetectorMARRESEARCH
Spacegroup nameP 31 2 1
Unit cell lengths74.970, 74.970, 196.900
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution39.226 - 3.702
R-factor0.2862
Rwork0.285
R-free0.31180
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3ze5
RMSD bond length0.005
RMSD bond angle0.795
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX ((PHENIX.REFINE))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]39.2303.800
High resolution limit [Å]3.7003.700
Rmerge0.0800.720
Number of reflections7069
<I/σ(I)>11.82
Completeness [%]96.798
Redundancy4.84.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1LIPIDIC CUBIC PHASE5.62773-5 %(V/V) 2-METHYL-2, 4-PENTANEDIOL, 0.1 M SODIUM CHLORIDE, 0.1 M LITHIUM NITRATE, 0.1 M SODIUM CITRATE/HCL PH 5.6. CRYSTALLIZED USING THE IN MESO (LIPIDIC CUBIC PHASE) METHOD AT 4 DEGREE CELSIUS WITH THE 7.8 MONOACYLGLYCEROL (7.8 MAG) AS THE HOSTING LIPID.

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PDB entries from 2024-05-15

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