3ZDM
Crystal structure of the Sgt2 N domain and the Get5 UBL domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2012-10-15 |
| Detector | BRUKER SMART 6500 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.109, 34.127, 92.092 |
| Unit cell angles | 90.00, 105.84, 90.00 |
Refinement procedure
| Resolution | 28.467 - 1.803 |
| R-factor | 0.1887 |
| Rwork | 0.187 |
| R-free | 0.22610 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.343 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.430 |
| Number of reflections | 37569 | |
| <I/σ(I)> | 27.2 | 4.2 |
| Completeness [%] | 97.8 | 97.2 |
| Redundancy | 7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M MIB BUFFER, PH 7.0 25% PEG 1500 |
