3ZDD
Structure of E. coli ExoIX in complex with the palindromic 5ov6 oligonucleotide and potassium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.359, 150.003, 34.228 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.340 - 1.500 |
| R-factor | 0.22071 |
| Rwork | 0.219 |
| R-free | 0.25367 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EXOIX FLAP1 FRAGMENT COMPLEX STRUCTURE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.624 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.150 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.520 |
| Number of reflections | 54849 | |
| <I/σ(I)> | 12.6 | 2.5 |
| Completeness [%] | 98.1 | 98.5 |
| Redundancy | 5.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 10 % ISOPROPANOL, 20% PEG 4000 |
