3ZD8
Potassium bound structure of E. coli ExoIX in P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM Q4 |
| Wavelength(s) | 0.870, 1.542, 1.542 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.370, 56.554, 60.489 |
| Unit cell angles | 110.35, 95.26, 94.88 |
Refinement procedure
| Resolution | 18.220 - 2.000 |
| R-factor | 0.16339 |
| Rwork | 0.160 |
| R-free | 0.22719 |
| Structure solution method | MIR |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.522 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.310 |
| Number of reflections | 34557 | |
| <I/σ(I)> | 8.8 | 2.1 |
| Completeness [%] | 92.3 | 80.9 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M SODIUM THIOCYANATE, 20% PEG 3350, 0.1 M MES AT PH 6.5 |
