3ZCT
Rabbit muscle glycogen phosphorylase b in complex with N-(2-naphthoyl) -N-beta-D-glucopyranosyl urea determined at 2.0 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2002-10-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.346, 128.346, 116.498 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.290 - 2.000 |
| R-factor | 0.18387 |
| Rwork | 0.183 |
| R-free | 0.20796 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qnb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.991 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.520 |
| Number of reflections | 65693 | |
| <I/σ(I)> | 23.3 | 4.6 |
| Completeness [%] | 99.2 | 98.6 |
| Redundancy | 6.5 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | pH 6.8 |
