3ZCJ
Crystal structure of Helicobacter pylori T4SS protein CagL in a tetragonal crystal form with a helical RGD-motif (6 Mol per ASU)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-31 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 197.122, 197.122, 106.808 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.965 - 3.250 |
| R-factor | 0.2075 |
| Rwork | 0.205 |
| R-free | 0.25130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zci |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.929 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.340 | 3.430 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.220 | 1.080 |
| Number of reflections | 33724 | |
| <I/σ(I)> | 8.1 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | PROTEIN CONCENTRATION: 10 MG/ML. RESERVOIR: 22.5% PEG 3350, 200 MM KCL. VAPOR DIFFUSION AT 277 K WITH DROP RATIO OF 1 TO 0.5 PROTEIN TO RESERVOIR. |
