3ZCI
Crystal structure of Helicobacter pylori T4SS protein CagL in a cubic crystal form with a distorted helical conformation of the RGD-motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97814, 0.97814, 0.97847 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 181.002, 181.002, 181.002 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.868 - 2.201 |
| R-factor | 0.2094 |
| Rwork | 0.207 |
| R-free | 0.24460 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.292 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCDE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.140 | 0.910 |
| Number of reflections | 24942 | |
| <I/σ(I)> | 12.7 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.4 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | PROTEIN CONCENTRATION:7 MG/ML. RESERVOIR: 100 MM MES PH6.5, 1.2 M AMMONIUM SULPHATE, 2-4 % 1,4 DIOXANE. VAPOR DIFFUSION AT 277 K WITH DROP RATIO OF |
