3ZC0
Structure of AfC3PO - duplex RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 183.060, 183.060, 198.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 83.091 - 2.982 |
| R-factor | 0.2103 |
| Rwork | 0.208 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zc1 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.242 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.780 | 3.060 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.110 | 0.830 |
| Number of reflections | 78522 | |
| <I/σ(I)> | 14.6 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.1 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2.7 M 1,6-HEXANEDIOL, 200 MM AMMONIUM CHLORIDE, 10 MM MGCL2, 50 MM HEPES PH 8.0, 5 MM DTT. |
