3ZBW
Crystal Structure of murine Angiogenin-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-18 |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.196, 95.673, 41.669 |
| Unit cell angles | 90.00, 100.99, 90.00 |
Refinement procedure
| Resolution | 27.679 - 1.801 |
| R-factor | 0.1681 |
| Rwork | 0.165 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bwk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.119 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 15155 | |
| <I/σ(I)> | 15.9 | 2 |
| Completeness [%] | 75.4 | 23.2 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 25% PEG 3350, 20MM SODIUM CITRATE; PH 4.6 AND 2% GLYCEROL |
