3ZBV

Crystal Structure of murine Angiogenin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-04
Detector	ADSC QUANTUM 4
Spacegroup name	I 41 2 2
Unit cell lengths	89.724, 89.724, 64.555
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.690 - 1.640
R-factor	0.19774
Rwork	0.198
R-free	0.20120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bwk
RMSD bond length	0.008
RMSD bond angle	1.228
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.740
High resolution limit [Å]	1.640	1.640
Rmerge	0.060
Number of reflections	29725
<I/σ(I)>	16.9	5.2
Completeness [%]	91.8	92.1
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		25% PEG 550 MME, 0.1M MES; PH 6.5 AND 0.01M ZNSO4