3X41
Copper amine oxidase from Arthrobacter globiformis: Product Schiff-base form produced by anaerobic reduction in the presence of sodium bromide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-13 |
| Detector | RIGAKU JUPITER 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.619, 63.290, 157.851 |
| Unit cell angles | 90.00, 117.21, 90.00 |
Refinement procedure
| Resolution | 31.881 - 1.870 |
| R-factor | 0.1529 |
| Rwork | 0.151 |
| R-free | 0.18020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.112 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.246 | 1.970 |
| High resolution limit [Å] | 1.700 | 1.870 |
| Rmerge | 0.102 | 0.399 |
| Number of reflections | 137175 | |
| <I/σ(I)> | 12.9 | 1.9 |
| Completeness [%] | 98.7 | 97.2 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES (pH6.8), MICRODIALYSIS, temperature 289K |
