3X3Y
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by histamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-12 |
| Detector | RAYONIX MX225HE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.471, 63.247, 157.906 |
| Unit cell angles | 90.00, 117.73, 90.00 |
Refinement procedure
| Resolution | 36.458 - 1.499 |
| R-factor | 0.1623 |
| Rwork | 0.162 |
| R-free | 0.17690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.078 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.499 | 1.500 |
| Rmerge | 0.088 | 0.425 |
| Number of reflections | 264870 | |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 98.1 | 97.4 |
| Redundancy | 6.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES (pH6.8), MICRODIALYSIS, temperature 289K |
