3X3X
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-28 |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.658, 62.890, 158.006 |
| Unit cell angles | 90.00, 117.48, 90.00 |
Refinement procedure
| Resolution | 20.414 - 1.570 |
| R-factor | 0.2163 |
| Rwork | 0.215 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.053 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.519 | 1.650 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.093 | 0.392 |
| Number of reflections | 228324 | |
| <I/σ(I)> | 16.7 | 1.9 |
| Completeness [%] | 98.1 | 96 |
| Redundancy | 6.6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES (pH6.8), MICRODIALYSIS, temperature 289K |
