3X3G
Fab fragment from anti TRAIL-R2 Human Agonist Antibody KMTR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 153.001, 165.383, 65.297 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.250 - 2.510 |
| R-factor | 0.18777 |
| Rwork | 0.186 |
| R-free | 0.22411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3x3f |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.701 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.300 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 27370 | |
| <I/σ(I)> | 27.6 | 3.8 |
| Completeness [%] | 95.4 | 81.1 |
| Redundancy | 6.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 20% PEG3350, 0.2M sodium sulfate, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
