3X38
Crystal structure of the C-terminal domain of Sld7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-13 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.094, 60.538, 93.130 |
| Unit cell angles | 90.00, 94.10, 90.00 |
Refinement procedure
| Resolution | 34.007 - 1.801 |
| R-factor | 0.2079 |
| Rwork | 0.206 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.029 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 35136 | |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 98.5 | 99.8 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M Acetate-NaOH, 0.1M LiSO4, 12.5% PEG 8K, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
