3WZT
Crystal structure of Trx3 domain of UGGT (detergent-unbound form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-08-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97888 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 196.400, 196.400, 196.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.400 |
| R-factor | 0.237 |
| Rwork | 0.235 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wzs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.279 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.127 | 0.677 |
| Number of reflections | 17411 | |
| <I/σ(I)> | 34.1 | 6.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 44.7 | 45.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 60% Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
