3WZS
Crystal structure of Trx3 domain of UGGT (detergent-bound form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-06-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.200, 93.600, 81.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.465 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.082 | 0.366 |
| Number of reflections | 19829 | |
| <I/σ(I)> | 47.9 | 7.2 |
| Completeness [%] | 98.5 | 98.9 |
| Redundancy | 6.8 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 23% PEG3350, 0.1M Tris-HCl (pH 7.0), 0.2M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
