3WYF
Crystal structure of Xpo1p-Yrb2p-Gsp1p-GTP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-06 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.490, 155.950, 149.820 |
| Unit cell angles | 90.00, 106.91, 90.00 |
Refinement procedure
| Resolution | 33.690 - 2.220 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m1i |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.695 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.690 | 2.260 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Number of reflections | 187333 | |
| Completeness [%] | 99.0 | 90.6 |
| Redundancy | 3.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 0.1M CITRATE, 17% PEG6000, PH 5.7, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
