3WXE
Crystal structure of CYLD USP domain (C596S) in complex with Met1-linked diubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-20 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.676, 49.788, 71.003 |
| Unit cell angles | 90.00, 102.69, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.18518 |
| Rwork | 0.182 |
| R-free | 0.24722 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2VHF and 1UBQ |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 15549 | |
| <I/σ(I)> | 2.9 | |
| Completeness [%] | 96.9 | 92.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 50mM Tris-HCl buffer, 9% PEG4000, 100mM magnesium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
