3WXB

Crystal structure of NADPH bound carbonyl reductase from chicken fatty liver

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 32 2 1
Unit cell lengths	74.568, 74.568, 190.925
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.590 - 1.980
Rwork	0.215
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yo6
RMSD bond length	0.025
RMSD bond angle	2.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.980
Number of reflections	43916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	23% PEG 5000 MME, 0.1M Bis-Tris buffer (pH 6.5) , VAPOR DIFFUSION, SITTING DROP, temperature 293K