3WVZ

Crystal structure of Hikeshi, a new nuclear transport receptor of Hsp70

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	90
Detector technology	AREA DETECTOR
Collection date	2013-11-08
Wavelength(s)	0.9192
Spacegroup name	C 2 2 21
Unit cell lengths	61.138, 137.839, 97.902
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.909 - 1.880
R-factor	0.2082
Rwork	0.207
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.865
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	100.000
High resolution limit [Å]	1.880	1.880
Number of reflections	19445

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293.15	0.1M Tris-HCl (pH 7.4), 1M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K