3WVT

Structural and biochemical study of equine lentivirus receptor 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-02
Detector	MAR CCD 165 mm
Wavelength(s)	0.97930
Spacegroup name	P 42 21 2
Unit cell lengths	81.139, 81.139, 42.274
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.491 - 1.601
R-factor	0.1952
Rwork	0.195
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fhq
RMSD bond length	0.006
RMSD bond angle	1.243
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.500	1.500
Number of reflections	19085
Completeness [%]	86.0	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	293	0.1M Citric acid, 25%(w/v) PEG3350, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K