3WVR
Structure of ATP grasp protein with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.600, 86.720, 94.140 |
| Unit cell angles | 73.87, 86.06, 68.23 |
Refinement procedure
| Resolution | 45.184 - 2.175 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.24600 |
| Structure solution method | MR-SAD |
| Starting model (for MR) | 3wvq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.156 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 2.300 | ||
| High resolution limit [Å] | 2.170 | 6.460 | 2.170 |
| Rmerge | 0.133 | 0.050 | 0.753 |
| Rmeas | 0.058 | 0.874 | |
| Number of reflections | 112313 | 4334 | 17318 |
| <I/σ(I)> | 8.04 | 22.62 | 2.08 |
| Completeness [%] | 97.4 | 98.7 | 92.8 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 293 | 0.1M Tris-HCl pH 8.1, 1.25M Lithium Sulfate, 10mM AMPPNP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
