3WU1

Crystal structure of the ETS1-RUNX1-DNA ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45PX
Synchrotron site	SPring-8
Beamline	BL45PX
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-25
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.02
Spacegroup name	C 2 2 21
Unit cell lengths	102.278, 140.902, 95.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.780 - 2.400
R-factor	0.213
Rwork	0.213
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1io4 1gvj
RMSD bond length	0.006
RMSD bond angle	1.330
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.049	0.352
Number of reflections	23925
<I/σ(I)>	13.1
Completeness [%]	87.4	65.1
Redundancy	4.9	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	11% PEG MME 550, 0.1M KCL, 0.015M MGCL2, 0.05M TRIS, pH 7.5, VAPOR DIFFUSION, temperature 298K