3WST
Crystal structure of C.elegans PRMT7 in complex with SAH(P31)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-04-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 |
Unit cell lengths | 190.703, 190.703, 373.088 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.410 - 2.390 |
R-factor | 0.23702 |
Rwork | 0.234 |
R-free | 0.30328 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.838 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.390 |
Number of reflections | 593769 |
<I/σ(I)> | 21.2 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 288 | 2M ammonium phosphate, 100mM Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 288K |