3WSR
Crystal structure of CLEC-2 in complex with O-glycosylated podoplanin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-02-04 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.369, 51.194, 54.099 |
| Unit cell angles | 90.00, 112.77, 90.00 |
Refinement procedure
| Resolution | 50.130 - 1.910 |
| R-factor | 0.21936 |
| Rwork | 0.217 |
| R-free | 0.25877 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 2C6U |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.368 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 21375 | |
| <I/σ(I)> | 24.3 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCl (pH 8.5), 25% (w/v) polyethylene glycol (PEG) 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
