3WSC

Crystal structure of alginate-binding protein Algp7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Detector technology	CCD
Collection date	2012-10-31
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	53.157, 97.704, 103.086
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.497 - 1.992
R-factor	0.2245
Rwork	0.223
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.711
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.990
Number of reflections	35629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.37	293	18% PEG5000MME, 0.1M Tris-HCl, 0.2M lithium sulfate, pH 7.37, VAPOR DIFFUSION, HANGING DROP, temperature 293K