3WRY

Crystal structure of helicase complex 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	84.981, 133.550, 195.786
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.750 - 2.300
R-factor	0.202
Rwork	0.202
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.750	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	99309
Completeness [%]	99.6	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	0.2M L-proline, 0.1M HEPES-KOH, pH7.0, 11%(w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, temperature 293K