3WRU
Crystal structure of the bacterial ribosomal decoding site in complex with synthetic aminoglycoside with F-HABA group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-27 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.554, 32.602, 51.413 |
| Unit cell angles | 90.00, 108.50, 90.00 |
Refinement procedure
| Resolution | 39.700 - 2.300 |
| Rwork | 0.249 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.921 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHENIX |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.700 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 6668 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Na Cacodylate, Spermine, KCl, MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
