3WRO

Minute virus of mice non-structural protein-1N-terminal nuclease domain reveals a unique Zn2+ coordination in the active site pocket and shows a novel mode of DNA recognition at the origin of replication

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Detector technology	PIXEL
Collection date	2014-02-20
Detector	PSI PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 2
Unit cell lengths	57.240, 121.350, 41.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.499 - 1.480
R-factor	0.1725
Rwork	0.171
R-free	0.19550
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	0.938
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
High resolution limit [Å]	1.480
Number of reflections	48534

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	293	2.8M sodium formate, 100mM sodium acetate trihydrate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K